番茄接种TYLCV后相关基因的表达分析
Expression Analysis of Related Genes After Inoculation of TYLCV in Tomato
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摘要: 以感TYLCV番茄品种"合作909大红番茄"和抗TYLCV番茄品种"华美204"为试材,采用荧光定量PCR技术,研究了TYLCV对感病、抗病番茄植株根、茎、叶片中水杨酸、茉莉酸和乙烯3条防御信号途径中关键因子的表达量的影响,以期了解番茄抗TYLCV的机制。结果表明:接种后30 d,感TYLCV材料"合作909大红番茄"各器官的TYLCV含量均显著高于抗TYLCV番茄材料"华美204",且根部含量最高,茎部次之,叶片含量最少。水杨酸途径相关基因PR1、NDR1、TGA2.1在叶片中"合作909大红番茄"的相对表达量显著低于"华美204",但在茎部和根部中高于"华美204"。NDR1和TGA2.1在根部的相对表达量最高,茎部次之,叶片最低。茉莉酸途径相关基因LoxD、Pin2在叶片和茎中"合作909大红番茄"的相对表达量低于"华美204",但在根部高于"华美204",LoxD、Pin2在根部的相对表达量最高。乙烯途径相关基因ERF3、EIN2在叶片中"合作909大红番茄"的相对表达量低于"华美204",但在茎部和根部中高于"华美204",ERF3和EIN2在根部的相对表达量最高,茎部次之,叶片最低。说明水杨酸、茉莉酸、乙烯3条信号传导途径共同参与了番茄抗TYLCV的过程。Abstract: TYLCV infected tomato variety ‘Cooperative 909 Red Tomato’ and TYLCV resistant tomato variety ‘Huamei 204’ were used as test materials, the effects of TYLCV on the expression levels of key factors in three defense signaling pathways in roots, stems and leaves of susceptible and resistant tomato varieties ‘Cooperative 909 Red Tomato’ and ‘Huamei 204’ were studied by fluorescence quantitative PCR,in order to understand the mechanism of tomato resistance to TYLCV.The results showed that 30 days after inoculation, the content of TYLCV in three organs of the TYLCV infected material ‘Cooperative 909 Red Tomato’ was significantly higher than that of TYLCV resistant material ‘Huamei 204’,and the content of root was the highest, followed by the stem, and the content of leaf was the lowest.The relative expression levels of salicylic acid pathway related genes PR1, NDR1 and TGA2.1 in leaves of ‘Cooperative 909 Red Tomato’ were significantly lower than ‘Huamei 204’,but higher than ‘Huamei 204’ in stems and roots.The relative expression levels of NDR1 and TGA2.1 were the highest in root, followed by stem and leaf.The relative expression levels of jasmonic acid pathway related genes LoxD and Pin2 in ‘Cooperative 909 Red Tomato’ were lower than ‘Huamei 204’ in leaves and stems, but higher than ‘Huamei 204’ in roots, with the highest relative expression levels in roots.The relative expression levels of ethylene pathway related genes ERF3 and EIN2 in leaves of‘Cooperative 909 Red Tomato’were lower than‘Huamei204’,but higher than‘Huamei 204’in stems and roots.The relative expressions of ERF3 and EIN2 were the highest in roots,followed by stems and the lowest in leaves.These results indicated the three signal transduction pathways of salicylic acid,jasmonic acid and ethylene were involved in the effect of tomato resistance to TYLCV.