Abstract: Taking ‘Ba Dao’ Gynostemma longipes as the test material which from Pingli county, Ankang, Shaanxi Province, and 8 kinds of substrates were set up with perlite, peat, garden soil, river sand and vermiculite as the raw materials according to different proportion.According to different treatments, the substrate characteristics（total porosity, water-holding porosity, aerated porosity）,rooting（initial rooting time, rooting rate） and sprouting growth（sprouting length, leaf number, biomass） characteristics, root morphological characteristics（average root diameter, total root surface area, total root length, total root volume） and leaf physiological and ecological characteristics（specific leaf weight, Pn, Gs, Tr, Ci） were measured regularly or continuously, to clarify the dynamic changing characteristics of Gynostemma longipes′ rooting and sprouting under different substrate treatments, to explore the influential mechanism of the substrate′s physical properties on Gynostemma longipes,and preliminarily select the appropriate cutting substrate and the best transplanting time range.The results showed that the sprout length of T₂（peat） treatment was significantly higher than other treatments（P＜0.05）,the total root length, total root surface area and total root volume were the highest, and the total root length and total root surface area were significantly higher than treatment without peat（P＜0.05） on the 24^th day of cutting.On this basis, it was also found that the specific root length, root length density and sprout length of cuttings were significantly correlated with the total porosity of the matrix（P＜0.05,R~2＞0.95）,and the sprout length was also significantly correlated with the specific root length and root length density（P＜0.05,R~2=0.99）.In Conclusion, the rooting and sprouting of Gynostemma longipes cutting affected each other, and the total porosity of the substrate was the main factor that significantly affected the root and leaf growth.The application of the substrate with higher total porosity could help shorten the cutting propagation time.In this study, peat could be used as the best substrate and the 12^th to 24^th day of cutting was suitable for transplanting.