Abstract: The factors affecting the induction of callus tissue, succession of callus tissue and differentiation of adventitious buds, succession of regenerating mass and of adventitious buds, and rooting of adventitious buds were investigated by using young leaves, petioles and root segments of the new variety ‘Black Queen’ as explants, and the annual yield of the seedlings was calculated by using mathematical formula, in order to provide reference for A.andraeanum moderate scale breeding and cultivation.The results showed that the best explants for callus tissue induction were leaves, followed by petioles and finally root segments, with induction rates of 70%,40% and 35% respectively; the best medium for callus tissue induction was 1/2MS+6-BA_0.5+2,4-D_0.5;the best medium for callus tissue succession and adventitious shoot differentiation was MS+6-BA_1.0+2,4-D_0.2.The best medium for the succession and adventitious shoot differentiation was MS+6-BA_1.0+NAA_0.2;the best medium for the rooting of clumped shoots was 1/2MS+NAA_0.2;the annual seedling yield mathematical formula of one bottle seedlings for the model of direct shoot, shoot and root segment production was Y=mXⁿ;the annual seedling yield mathematical formula of one bottle seedlings for the model of regenerating mass or callus tissue for shoot production was Y=ac（1-Xⁿ）/（1-X）.