Abstract: Taking A.mongholicus seedlings as experimental materials,the effects of cultivated and imitating wild on the estimation methods of astragaloside and calycosin-7-glucoside content in A.mongholicus were studied by using hyperspectral and multiple linear regression methods,in order to provide reference for the rapid and accurate estimation of the medicinal composition content of cultivated and imitating wild A.mongholicus in practice.The results showed that the stability and the fitting degree of the models established by the raw hyperspectral data of medicinal composition content of cultivation and imitating wild A.mongholicus were the best.The RMSE of the models to estimate the astragaloside content were 0.004 5,0.008 5;the R~2 were 0.761,0.879;the models were y=0.005 4+0.001 6x₁+0.000 2x₂+0.000 7x₃（R~2=0.903）,y=-0.122 3+0.004 0x₁+0.000 1x₂-0.000 9x₃（R~2=0.904）,respectively.The RMSE of the models to estimate the calycosin-7-glucoside content were 0.001 7,0.004 0;the R~2 were 0.860,0.868;the models were y=0.073 1-0.008 0x₁-0.000 4x₂+4×10^-6 x₃（R~2=0.891）,y=0.084 2-0.000 7x₁-0.000 1x₂+0.000 2x₃（R~2=0.883）,respectively.