Abstract: Naturally diseased fruit of ’Jinshi No.4’ kiwifruit were used as test material.The fungi were isolated from a large number of rotten samples,and were identified through their morphologic characteristics and molecular analysis.Koch’s postulate was performed to confirm the identification.The fungi which cause post-harvest stem-end rot of yellow-flesh kiwifruit was identified,in order to provide reference for the research on the prevention and control of post-harvest rot disease of yellowflesh kiwifruit.The results showed that eight different fungi were causable for stem-end rot of kiwifruit.They were Phomopsis sp., Diaporthe cotoneastri,Pestalotiopsis microspora,Botrytis cinerea,Neofusicoccum parvum,Alternaria sp.,Fusarium sp.and Botryosphaeria dothidea.