Abstract: ‘Blue Mouse Ears’ Hosta was used as the research material.The effects of different light quality and light intensity on the growth and rooting of tissue culture plantlets were studied by LED,the suitable light quality and light intensity were selected, in order to provide reference for the industrialized production of ‘Blue Mouse Ears’ Hosta plantlets.The results showed that, 1)when the LED light quality was W∶R=7∶1,the proliferation rate of tissue culture plantlets was 4.71,which was the highest among all treatments; the comprehensive performance of plant height and leaf area was good, which were 17.48 mm and 30.90 mm~2 respectively, indicated that W∶R=7∶1 could promote the growth of tissue culture plantlets.Treatments of R∶B=5∶1,W∶R=7∶1 and R∶W=2∶3 had a rooting rate higher than 97%,which were better than other treatments.When the LED light quality was R∶W=2∶3,the root number of plantlets was 17.54,which indicated that the light quality R∶W=2∶3 could promote the root growth of tissue culture plantlets effectively.2)The lowest light intensity 59 μmol·m^-2·s^-1 was beneficial to the proliferation of tissue culture plantlets, with the increase of light intensity, the proliferation coefficient decreased.When the light intensity was 176-235 μmol·m^-2·s^-1,the leaf area of tissue culture plantlets were good, and the numerical value was 73.09 mm~2 respectively, indicated that the light intensity of 176-235 μmol·m^-2·s^-1 could promote the growth of tissue culture plantlets.When the light intensity was 118-176 μmol·m^-2·s^-1,the maximum root length was 7.44 mm, which indicated that the light intensity in the range of 118-176 μmol·m^-2·s^-1 could promote the root growth of tissue culture plantlets.The lowest light intensity(0-59 μmol·m^-2·s^-1) and the highest light intensity(706-824 μmol·m^-2·s^-1) were not conducive to the growth of plantlets.