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碱性盐胁迫对节瓜幼苗耐盐生理特性的影响

Effects of Salt-alkaline Stress on Physiological Characteristics of Salt Tolerance in Chieh-qua Seedlings

  • 摘要: 以‘菲绿达1号’节瓜为试材,采用水培的方法,设置不同的碱性盐NaHCO3(0、25、50、75、100mmol·L-1和125mmol·L-1)处理,研究了不同浓度碱性盐胁迫对节瓜生理特性的影响,以期为碱性盐胁迫对节瓜的胁迫损伤机理提供参考依据。结果表明:25、50mmol·L-1等低浓度盐胁迫对节瓜幼苗的影响不显著,但较高浓度的盐胁迫使节瓜幼苗的光合系统出现损伤,Y(Ⅱ)、ETR、q P、Fv/Fm出现显著上升。此外,高浓度的盐胁迫使得节瓜的活性氧含量(■和H2O2)和多种抗氧化酶活性显著上调;盐胁迫还使丙二醛(MDA)含量和相对电导率显著上升,这可能是ROS过量积累引起的;研究还发现高浓度的盐胁迫还促进了脯氨酸、可溶性糖和可溶性蛋白质等渗透调节物质的显著上升。综上所述,25、50mmol·L-1盐溶液对节瓜幼苗的损伤有限,但75mmol·L-1及以上的盐浓度超出节瓜幼苗的耐受范围;此外,节瓜主要通过增加光合热耗散、提高抗氧化酶活性和增加渗透调节物的合成来应对盐胁迫。

     

    Abstract: Chieh-qua seedlings ‘Feicuida 1’ was selected as the experimental material, the method of hydroponics was adopted and different salt concentrations(0,25,50,75,100,125 mmol·L-1) were set up to observe and measure the physiological response of chieh-qua seedlings, in order to provide reference for the damage mechanism of alkaline salt stress on chieh-qua seedlings. The results showed that low concentrations of salt stress such as 25,50 mmol·L-1 had no obvious effect on the seedlings, but higher concentrations of salt stress caused damage to the photosynthetic system of the seedlings, and Y(Ⅱ),ETR,qP,Fv/Fm increased significantly.In addition, the ROS content ■ and the activities of various antioxidant enzymes were significantly up-regulated under high concentration salt stress.Salt stress also significantly increased MDA content and relative conductivity, which may be caused by excessive accumulation of ROS.Finally, the study also found that the high concentration of salt stress also promoted the significant increase of osmoregulatory substances such as Pro, soluble sugar and soluble protein.In conclusion, the damage of 25,50 mmol·L-1 salt solution was limited, but the salt concentration of 75 mmol·L-1 and above exceeded the tolerance range of chieh-qua seedlings.In addition, the chieh-qua could cope with salt stress by increasing photosynthetic heat dissipation, increasing antioxidant enzyme activity and increasing osmoregulator synthesis.

     

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