Abstract: The variety of ‘Maitiansanpinqing’ asparagus lettuce was used as the test plant.The method of foliar spraying was used.The effects of growth regulator combinations,promoter-inhibitors-promoter-inhibitors（T1）,promoter-CCC-promoter-CCC（T2）,GA₃-inhibitors-GA₃-inhibitors（T3）,GA₃-CCC-GA₃-CCC（T4）,GA₃-GA₃-GA₃-GA₃（T5） and spraying water（CK） on growth and photosynthesis of ‘Sanqing’ asparagus lettuce were studied.In order to screen out a combination of plant growth regulators that could promote the efficient production of ‘Sanqing’ asparagus lettuce.The results showed that different growth regulator combinations increased the plant height,stem diameter,leaf number,per plant weight of asparagus lettuce.In the prophase,the plant height,stem diameter,leaf number,per plant weight of T5 grew faster than other treatments,and the rate of growth decreased in the later period.When the promoter and inhibitor were used alternately,the growth of them was slower in the prophase,but they increased faster and greater than T5 in the later period.The stem diameter,leaf number,per plant weight of T2 and T3 were greater than other treatments.The chlorophyll content of T2 was the largest,and it significantly increased compared with CK.Therefore,the best combination of growth regulators was T2,which improve asparagus lettuce photosynthesis and promotes lettuce growth.