秦岭石蝴蝶叶片离体组织培养技术

胡选萍; 蒋景龙; 王琦; 李耘

秦岭石蝴蝶叶片离体组织培养技术

In Vitro Tissue Culture Technology of Leaves of Petrocosmea qinlingensis

摘要

摘要: 以秦岭石蝴蝶（Petrocosmea qinlingensis）幼嫩叶片为试材，采用离体组织培养方法，研究了外植体消毒、初代培养、愈伤组织继代分化、不定芽增殖、生根炼苗对秦岭石蝴蝶离体再生的影响，以期为建立秦岭石蝴蝶组织培养快繁体系提供参考依据。结果表明：0.1%氯化汞处理2 min+无菌水洗涤7～8次为最适灭菌方法；最适愈伤组织诱导与继代培养基为MS+2.0 mg·L^-1 IBA;愈伤组织分化的合适培养基为MS+0.1 mg·L^-1 6-BA+0.2 mg·L^-1 NAA+0.1 mg·L^-1 IBA;正交实验结果表明MS+0.1 mg·L^-1 6-BA+0.1 mg·L^-1 IBA+0.1 mg·L^-1 ZT为最适增殖培养基，最大增殖系数达14.06;最适生根炼苗条件为珍珠岩、蛭石和泥炭（2∶1∶1）基质+MS营养液1 mL+200 mg·L^-1 IBA 0.5 mL。

Abstract: Taking leaves of Petrocosmea qinlingensis as test materials, the effects of explants disinfection, primary culture, subculture and differentiation of callus, adventitive bud multiplication, rooting and seedling training on regeneration of Petrocosmea qinlingensis were studied by using in vitro tissue culture method, in order to provide reference for the establishment of rapid propagation system of Petrocosmea qinlingensis.The results showed that optimal disinfection method was treatment with 0.1% mercuric chloride 2 minutes and wash with sterile water 7—8times.Best medium for callus induction and subculture was MS+2.0mg·L-1 IBA.Suitable medium for callus differentiation was MS+0.1mg·L-1 6-BA+0.2mg·L-1 NAA+0.1mg·L-1IBA.The results of orthogonal experiment indicated that the proper condition for adventitious buds induction was MS+0.1mg·L-1 6-BA+0.1mg·L-1 IBA+0.1mg·L-1 ZT and maximum multiplication coefficient up to 14.06.Optimum rooting and growing cultivation conditions was substrate of perlite,vermiculite and peat（2:1:1）+MS nutrient solution（1mL）+200mg·L-1 IBA（0.5mL）.

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