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种子引发对干旱胁迫下黄瓜生理及品质的影响

Effects of Seed Priming on Physiology and Quality of Cucumber Under Drought Stress

  • 摘要: 以‘绿岛7号’黄瓜为试验材料,用5%PEG对种子进行4 h引发处理,以未引发种子为对照,设置4个干旱梯度,采用双因素裂区分析方法,探究PEG引发对黄瓜生理及果实品质的影响,以期为PEG作为黄瓜种子引发剂或包衣剂物料筛选提供参考依据。结果表明:PEG引发的植株超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性均有提高,叶绿素含量显著增加了22.18%~37.99%,在最大田间持水量的40%~50%时净光合速率提高了118.60%;PEG引发的黄瓜产量高于未引发,在最大田间持水量的50%~60%时产量低于最大田间持水量为60%~70%,但差异不显著,且在最大田间持水量的50%~60%时,PEG引发的果实在可溶性固形物、可溶性糖、可溶性蛋白质等品质上有所提高,维生素C较未引发显著提高了75.38%,说明有效的PEG引发可以通过增强干旱胁迫下植株的抗氧化酶活性、叶绿素含量、光合指标等来提高果实品质。

     

    Abstract: Taking ‘Lyudao No.7’ cucumber as experimental material, the seeds were induced by 5% PEG for four hours, and the seeds were not induced by 5% PEG as the control.Four drought gradients were set, and two-factor splitting analysis method was used to explore the effects of PEG on cucumber physiology and fruit quality, in order to provide reference basis for the selection of cucumber seed priming or coating agent materials with PEG.The results showed that PEG induced plant superoxide dismutase(SOD),peroxidase(POD),and catalase(CAT) activity were increased, chlorophyll content was significantly increased by 22.18%-37.99%,and net photosynthetic rate of 40%-50% maximum field water capacity was increased by 118.60%.The yield of cucumber induced by PEG was higher than that of cucumber not induced by PEG,and the yield at 50%-60% of the maximum field capacity was lower than 60%-70% of the maximum field capacity,but the difference was not significant.At 50%-60% maximum field capacity,soluble solids,soluble sugar,soluble protein of cucumber induced by PEG was higher than that of cucumber without PEG.Vitamin C were significantly increased by 75.38% compared with those without PEG induction,suggesting that effective PEG induction could improve fruit quality by enhancing antioxidant enzyme activity and chlorophyll content and photosynthetic index of plants under drought stress.

     

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