Abstract: Taking pepper breeding resources 9-68 as the research object, cDNA-AFLP technology was used to analyze different developmental stages of pepper fruits, and bioinformatics analysis was conducted to explore the differential expression of related genes during pepper fruit development, in order to obtain relevant genes and expression information for pepper fruit development.The results showed that a total of 1 926 TDFs（transcriptive derived fragments） were amplified using 42 primer combinations, including up-regulated expression, down-regulated expression, and undifferentiated expression types.Selecting 25 specifically expressed TDFs for recovery sequencing, 19 clear sequence results were obtained.Among them, 8 TDFs sequences were found in the Blastn library to have known cDNA,mRNA,or gene sequences with high similarity, while 8 TDFs sequences were found in the Blastx library to have cDNA or mRNA sequences with high similarity that had been registered in the database, but their specific functions had not been determined.In the comparison analysis of three TDFs in GeneBank and EST libraries, no results with high similarity were found, which may be the first discovered base sequence.