Abstract: Iris ensata seedlings were used as the test materials.The potted method was used to study the effects of different copper solution stress treatments on the growth and physiological metabolism of Iris ensata seedlings,in order to provide a reference for further understanding of the toxic mechanism of heavy metal copper on the growth of Iris ensata.The results showed that the growth and physiological indicators of Iris ensata seedlings treated with different copper solution concentrations were significantly different.When the copper solution concentration was 50 mg·kg-1（T1）,it could promote the photosynthesis of plants and accelerate the growth of seedling leaves.However,with the increase of the copper ion concentration and the extension of the treatment time（T2-T5）,the plant stress response was obvious,photosynthesis was inhibited,the active oxygen in the plant accumulated too much.SOD activity rised to the highest and then dropped.Content of MDA,relative conductivity and osmotic adjustment substance continued to rise.Eventually copper stress destroyed the physiological environment of the plant body,resulted in poisonous damage to the leaves and roots of the Iris ensata.