Abstract: In order to identify the pathogens of root rot of tree peony,infected roots were used as test materials from different cultivated fields in Luoyang from 2016 to 2018.The pathogenic isolates were obtained and purified by tissue isolation methods.The pathogenicity was proved by the Koch′s rule.The pathogenic isolates were identified based on morphological characteristics and gene sequencing analysis.The results showed that fifteen isolates were obtained from 30 samples of root rot.The results of pathogenicity test showed that nine isolates could infect tree peony roots.The pathogens isolates were identified preliminary as Fusariumbased on morphological characteristics.The results of rDNA-ITS and EF-1αgene sequencing analysis showed that seven isolates were identified as Fusarium solani and two isolates were identified as Fusarium oxysporum.Based on the above results,it was concluded that root rot of tree peony was caused by both F.solani and F.oxysporumin Luoyang,and F.solani was dominant pathogen.