Abstract: Taking Diospyros lotus L.tissue cultured seedlings as test material, the effects of the different plant growth regulator treatment on the tissue cultured seedlings root were studied, and the different moisturizing time after transplanting the influence of tissue seedlings transplanting survival rate were explored, in order to obtain Diospyros lotus L.tissue seedlings root transplanting efficient technology system and provide reference for the clonal propagation of persimmon rootstocks.The results showed that, the group culture seedling had the best rooting effect at 1.0 mg·L^-1 IBA+0.5 mg·L^-1 IAA treatment.The rooting rate could reach 95.56%,the average number of root bars was 7.93,the average fresh weight was 0.59 g, root system vitality of 0.99 μg·g^-1·h^-1,the average total root length was 26.57 cm, the average root surface area was 10.33 cm~2,the average root volume was 0.32 cm~3,the average root diameter could reach 0.61 mm.The highest survival rate of 10 days moisturizing culture after transplanting was 90.44%.The average plant height was 64.32 cm at 5 months after transplanting to the field, stem thick, 6.86 mm, could be used for the grafting and propagation of the scions.