Abstract: Eight A.auricula strains as materials, the optimal medium is selected from PDA medium, bran medium, corn flour medium and PDA improved medium based on the mycelial growth status, growth time and the biomass of solid strains（different pH） and liquid strains, and the culture conditions of high-quality strains in the optimal medium were optimized.The results showed that the mycelium of XLG-1,XLG-2,XLG-5 and XLG-6 grew faster and they had lower pollution rate and stronger mycelial growth potential than others, in PDA solid medium.Among the mycelium of XLG-1,XLG-2,XLG-5 and XLG-6,XLG-1 and XLG-2 grew faster than others with pH 6 and 9,respectively, in PDA solid medium.The mycelial biomass of XLG-1 and XLG-2 from PDA improved medium was significantly higher than that from other media.The optimum culture conditions of XLG-1 and XLG-2 in PDA improved medium were as follows, 100 mL of medium loading, 25 ℃ of temperature and 10% of inoculation amount.In conclusion, XLG-1 and XLG-2 had strong adaptability, fast growth rate and large biomass.PDA improved medium was the best medium.XLG-2 was a high-quality strain with high pH resistance and was suitable for cultivation in Southern Xinjiang.