Abstract: 18 Lycoris spp.resources were used as materials to study the ornamental characteristics, ploidy and the effects of hybrid combinations on seed setting rate by periodic observation and flow cytometry, in order to provide basis for the breeding of new varieties with independent intellectual property rights.The results showed that there were differences in ornamental characters among different Lycoris spp.resources.The flower colors included bright red, blue purple, white and yellow.Some resources, such as Lycoris longituba and Lycoris chinensis had 56.41-62.24 cm long scape and strong flowers, which were good materials for cut flowers.By analyzing the ploidy of Lycoris spp.resources, 12 diploid and 6 triploid germplasm were identified.According to the principle of trait specificity and trait complementary, 64 cross combinations were designed, and the results were statistically analyzed.The results showed that the Lycoris sprengeri,Lycoris chinensis,Lycoris haywardii and Lycoris longituba had good cross seed setting rate, which was a good parent material for subsequent cross breeding.