Abstract: A NAC（NAM,ATAF1/2,CUC2） transcription factor gene DcNAC48 was cloned by PCR from Dendrobium catenatum Lindl..The characteristic structures of the gene were analyzed by bioinformatics and the expression patterns in different tissues and under different stresses were studied by quantitative real-time PCR（qRT-PCR）,in order to lay a foundation for further study of the function of DcNAC48 and provide gene resources for improving the stress resistance of D.catenatum.The results showed that the total length of DcNAC48 gene was 909 bp, encoding 302 amino acids, and had a conserved NAC domain.There were various hormone responsive elements, stress responsive elements, growth and development responsive elements and light responsive elements in the gene promoter region.The expression patterns analysis showed that DcNAC48 had tissue specificity, and its expression level was the highest in the gynostemium.The gene expression patterns under different stress treatments indicated that DcNAC48 was upregulated by low temperature, salt stress, drought stress and high temperature stress, suggesting that DcNAC48 might be involved in the response of D.catenatum Lindl.to abiotic stress.