Abstract: Taking the fruit of blueberry variety ‘Baldwin’ as the material, Unigenes was obtained by high-throughput sequencing, SSR information analysis was analyzed, and blueberry EST-SSR functional marker primers were developed, in order to provide reference for the molecular biological mechanism of gene mapping and fruit quality formation of blueberry.The results showed that, a total of 73 468 Unigenes were obtained by transcriptome sequencing, of which the frequency of SSR was 20.81%,and 3 354 SSR sites containing sequences, were obtained.The average length of transcriptome SSRs in blueberry fruit was 20 bp and the average distribution frequency was 1/3.90 kb.In the blueberry transcriptome SSR,the proportion of Dinucleotide was the highest（41.02%）,followed by mononucleotide.The blueberry transcriptome SSR of a single SSR site contains 173 repeat nucleotides, and A/T（36.56%） and AG/CT（41.03%） were the dominant repeat unit types, respectively.Unigene containing SSR sites was enriched into 57 GO terms of three ontology categories, of which the most GO terms involved in reproduction, binding and multicellular biological processes.Based on the transcriptome information analysis of blueberry fruit, 82 pairs of SSR sequence primers were finally designed.The development of these potentially polymorphic SSRs might have high application value in blueberry genetic breeding research and resource evaluation.