Abstract: Taking the pathogen of Morchella white mold disease in Hexi Area as the experimental materials, the pathogen of Morchella white mold was isolated and purified by tissue separation method.The pathogen was identified by morphological and molecular biological methods, and the effects of different temperature, pH, carbon source, nitrogen source and medium on the growth of the pathogen were clarified, in order to provide reference for the development of effective prevention and control measures of morel of Morchella white mold.The results showed that the pathogen was Diploospora longispora based on morphological and ITS sequence analysis.The biological characteristics showed that the suitable growth temperature range of the pathogen was 20-25 ℃,and the pathogen stopped growing above 30 ℃.The pathogen grew well in acidic environment, and the optimum growth pH was 6.0-6.5.The optimum carbon source, nitrogen source and medium for growth were sucrose, peptone and PCA medium, respectively.