Abstract: In this study,10 strains of Lyophyllumfumosum were used as materials,and their relationship and growth characteristics were studied through antagonism test,solid and colony growth test in liquid medium and agronomic character cultivation test,in order to provide a reference for the industrial cultivation of Lyophyllumfumosum.The results showed that strains A,B,E,F and I were identified as independent strains by antagonism test,and strains C,D,J,G and H were the same strain.The comprehensive characters of strain F was the best.The mycelium growth was even and the mycelium growth speed was the fastest,reaching（4.45±0.01）mm·d-1 when cultured on solid medium,and the density of fungus ball was the highest,reaching 188 pellet·mL^-1,the diameter of fungus ball was the shortest,only 1.23 mm with the biomass of 253 mg·（10 mL）-1 when cultured in liquid medium.In fruiting cultivation experiment,strain F had the lowest contamination rate and grew tidily,the biological efficiency was significantly higher than that of other strains,reaching 55%.