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混合盐碱胁迫对孔雀草生长的影响

Effects of Mixed Saline-alkali Stress on Growth of Tagetes patula

  • 摘要: 以孔雀草种子和幼苗为试材,通过模拟山西省运城市盐湖周边环境特征,采用NaCl、Na2SO4 2种中性盐和NaHCO3和Na2CO3 2种碱,按照1∶9∶9∶1摩尔比进行混合,将混合好的盐碱按浓度梯度设置为25、50、75、100、125、150、200、300 mmol·L-1 8个浓度,研究了不同浓度混合盐碱对孔雀草种子和幼苗的影响,以期为盐湖区域孔雀草种植及引种驯化提供理论依据。结果表明:孔雀草种子发芽率、发芽势、发芽指数和活力指数随着混合盐碱处理液浓度升高而逐渐降低。胚根、胚芽、胚轴和子叶面积随胁迫浓度升高而呈现缩小趋势。胁迫后幼苗叶绿素、相对电导率随胁迫浓度升高呈现上升的趋势。丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性随胁迫浓度升高而呈现先升高后降低趋势。游离脯氨酸(Pro)含量随胁迫浓度升高而逐渐升高。随着浓度增加,盐碱胁迫对孔雀草种子和幼苗影响越大。

     

    Abstract: The effects of different concentrations of mixed saline-aslkali on seeds and seedlings of Tagetes patula were studied in order to provide theoretical basis for the planting,introduction and domestication of Tagetes patula in salt lake area.By simulating the environmental characteristics around salt lake in Yuncheng city,Shanxi Province,two kinds of neutral salts of NaCl and Na2SO4,two kinds of alkali of NaHCO3 and Na2CO3 were selected and mixed according to 1:9:9:1 mole ratio.The concentrations of mixed saline-alkali that NaCl,Na2SO4,NaHCO3 and Na2CO3 were set to25,50,75,100,125,150,200 mmol·L-1 and 300 mmol·L-1 solution.The results showed that the germination rate,germination energy,germination index and activity index of Tagetes patula gradually decreased;the area of embryo root,germ,hypocotyl and cotyledons decreased;chlorophyll content and relative conductivity of seedlings showed an rising trend;the content of MDA,the activities of SOD and CAT increased and then decreased;the content of free proline(Pro)gradually increased with the increase of the concentration of mixed saline-alkali treatment solution.The greater the concentration of mixed saline-alkali,the more the influences on the seeds and seedlings of Tagetes patula.

     

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