Abstract: Taking 127 typical walnut orchards which located in northwestern high altitude cold areas, northern high latitudes, southwestern and southern high altitude areas of Guizhou as test materials, the contents of nitrogen（N）,phosphorus（P）,potassium（K）,calcium（Ca）,magnesium（Mg）,iron（Fe）,manganese（Mn）,copper（Cu）,zinc（Zn） and boron（B） in leaves were determined.The leaves′ mineral nutrition was diagnosed by the method of percentage deviation of suitable value（DOP）,in order to provide a reference for scientific fertilization in the cultivation of J.sigillaia in Guizhou.The results showed that the appropriate value range of leaves mineral elements in walnut main cultivation of Guizhou was as follows, N 2.38%-2.84%,P 0.27%-0.39%,K 0.69%-1.07%,Ca 2.44%-3.08%,Mg 0.67%-0.91%,Fe 162.15-235.67 mg·kg^-1,Mn 132.92-204.52 mg·kg^-1,Cu 13.81-17.71 mg·kg^-1,Zn 20.41-28.93 mg·kg^-1,B 32.61-46.85 mg·kg^-1.Among all the elements, B and K were extremely deficient, N was relatively deficient, Zn was basically abundant, Mn, Cu, Ca, Mg and P were sufficient, and Fe had differences among areas.The nutrition imbalance index（NII_m） showed that the mineral elements in leaves of the four main walnut producing areas were all imbalance.The balance of southwestern area was the best, and that of the other three areas was not different.In conclusion, it was in urgent need to use B and K fertilizer, N fertilizer management was also need to strengthen, and Mn, Cu and Ca fertilizer should be controlled in all walnut production areas, however, Fe fertilizer should be additional application in southern of Guizhou.At the same time, it was also need to improve the balance of leave′s mineral elements in the walnut orchard of Guizhou Province.