Abstract: ‘Fujiu No.10’ was taken as the experimental material, different nitrogen treatments were set up after planting for one month, the growth, physiology, yield and quality indexes of Chinese chives were determined during the vigorous growth period（6 months）,in order to explore the effects of different nitrogen application on the growth, physiology, yield and quality of Chinese chive.The results showed that with the increasing of nitrogen application, the external morphology（pseudo stem diameter, leaf length, leaf width, leaf thickness）,biomass accumulation and yield（the first crop） of Chinese chive first increased and then decreased.The Chinese chive of N4 and N5 treatments had the best growth, higher fresh and dry weight and yield.According to the fitting equation, the highest yield per 667 m~2 of the first crop of chive and its corresponding nitrogen application respectively were 2 591.4 kg and 18.537 kg.With the increasing of nitrogen application, the root activity of Chinese chive first increased and then decreased, and the N3 had the strongest root activity; The content of chlorophyll（Ca, Cb） in N4 was the highest, followed by N5.On the whole, the change trend of photosynthetic pigment and root activity was basically consistent with the growth status and yield of Chinese chive.With the increasing of nitrogen application, the Pro content, MDA content, SOD activity and POD activity of Chinese chive decreased at first and then increased, the N4 showed the least stress level.The contents of soluble sugar, vitamin C,soluble protein and titratable acid in Chinese chive increased at first and then decreased with the increasing of nitrogen application, the effect of nitrogen application on the soluble protein content of Chinese chive was not obvious, the nitrate content of Chinese chive increased with the increasing of nitrogen application.The soluble sugar content of N5 was the highest, and the vitamin C and titratable acid content of N3 were the highest.According to the growth, physiology, yield and quality of Chinese chive, the nitrogen application rate of N4 and N5 were showed better.That is, the average monthly nitrogen application rate per 667 m~2 was 36.84-45.96 kg, which could be used as a reference for the nitrogen application rate of Chinese chive under greenhouse cultivation conditions.