Abstract: Species resources of macrofungi in Pingbian county of Yunnan Province were investigated by field treading survey, in order to provide reference for the effective protection, rational development and utilization of macro fungi resources in this area.The results showed that there were 319 species of macrofungi in Pingbian country, belong to 6 classes, 17 orders, 50 families and 114 genera of Ascomycota and Basidiomycota.3 new records of Yunnan, Cortinarius semisanguineus（Fr.） Gillet, Phellinus alni（L.） Quél.and Tyromyces sibiricus（Penzina ＆ Ryvarden） Spirin were discovered.In terms of family and genus composition, the dominant families were 9 families, including 186 species, accounting for 58.31% of the total species, including Russulaceae, Boletaceae, Polyporaceae, etc.The dominant genera were Russula,Amanita,Lactarius and total 17 genera, including 166 species, accounting for 52.04% of the total species.According to the data analysis, the economic types of macrofungi were abundant in Pingbian country.There were 82 species of edible fungi, 34 species of medicinal fungi, 29 species of toadstool, and 10 species were both used for edible and traditional medicine.The species of edible macrofungi accounted for 25.71% of the total species.