Abstract: Taking strawberry as material, the characters of plant and fruit were investigated in field plot and greenhouse control experiments, so as to determine and define the effect on growth and quality of strawberry by applying gongzhulingmycin.The results showed that transplanting survival rate of ‘Gongzhu No.4’ strawberry and ‘Gongzhu No.3’ strawberry were 82.46% and 84.57%,which were higher than the control as the ratio of 32.68% and 18.15% respectively in the pouring root treatments with the water extract of gongzhulingmycin after transplanting.The characters of plant height, petiole length, leaf length, leaf width, the leaf number, crown diameter were all better than the control, and the leaf size and leaf number were more significant than the control in the treatments.The optimum application concentration for pouring root was 100× during the process of transplanting.The phenological period of strawberry were not significant among the treatments of spraying, pouring and mixed method of them, but the method of pouring could improve the character of fruit more significant than other two methods.The maximum fruit weight and average fruit weight of strawberry were improved by the ratio of 12.18% and 13.79% higher than those of the control, respectively.The soluble solids content and fruit firmness were increased significantly.The results showed that application of gongzhulingmycin can improve the growth and quality of strawberry in field.