Abstract: Taking Rehmannia glutinosa as material and pearl rock∶vermiculite（3∶1） as a culture matrix.The HPLC fingerprint spectrum and OPLS-DA analysis methods were carried out.Extraction of the total phenylethanoid glycosides and the total iridoid glycoside were used as treatment liquid.Three groups of total phenylethanoid glycosides treatment group, total iridoid glycoside treatment group, and blank group were used for indoor controlled experiments.The effects of total phenylethanoid glycosides and the total iridoid glycoside on the growth and quality characteristics of Rehmannia glutinosa were studied, in order to provide reference for the effect of secondary metabolites products on growth and quality characteristics of Rehmannia glutinosa.The results showed that the total phenylethanoid glycosides and the total iridoid glycosides had a degree of promoting Rehmannia glutinosa growth and root development.Acteoside and isoacteoside might be an important component of regulating the growth of leaves.Echinacoside, hydroxytyrosol and ajugol might be one of the important components of regulatory root growth.