Abstract: Taking the diseased fruit of cherry brown rot from Guizhou as experimental materials, the pathogen causing cherry brown rot was verified based on Koch postulates, and identified based on morphological and physiological, and their biological characteristics was explored, in order to provide reference for the accurate prevention and control of cherry brown rot.The results showed that the isolated pathogenic strain YTBH10 had a grey-brown or grey-white colony with a large number grey-brown spores which were concentric wheel shape and conidia were arranged in chain shape.Phylogenetic tree based on ITS indicated that the strain belonged to Monilinia.The candidate isolate could cause disease after experiment on cherry fruits.Therefore, the pathogen causing brown rot in Guizhou cherry was identified as Monilinia fructicola.The pathogen could grow in the pH range of 3-13 and prefered a neutral to slightly acidic environment with the optimal temperature for mycelial growth being 25 ℃.