Abstract: The drought resistance of grape rootstocks 110 R,1103 P,5 BB were used as materials,the effects of drought stress on photosynthetic characteristics and anti-oxidation ability of grape rootstocks were studied by using conventional physical and chemical index determination methods.In order to evaluate its resistance.The results showed that the stomatal conductance,transpiration rate and photosynthetic rate of the three rootstocks decreased with the drought stress, and the intercellular CO₂ concentration decreased first and then increased.The maximum photochemical efficiency of PSⅡ and the actual photoenergy conversion efficiency of PSⅡ in rootstock leaves showed a decreasing trend. The content of malondialdehyde（MDA） showed a rising trend, and the rising range of 5 BB was the biggest, and the rising range of 110 R was the smallest.The content of free proline showed a rising trend, and the rising range of 110 R was the biggest,the rising range of 5 BB was the smallest.The leaf catalase activity of three grape rootstocks showed a rising trend, and the rising range of rootstock 110 R was the biggest,and the rising range of 5 BB was the smallest.On the 20^th day of drought stress,the relative water content in the leaves of rootstock 110 R was higher than that of rootstock 5 BB.In conclusion,the drought resistance of rootstocks was 110 R＞1103 P＞5 BB.