Abstract: A novel antimicrobial peptide（AMP-17） prepared by inductive expression was used as the research object to determine its antimicrobial activity against several food spoilage microorganisms by plate diffusion method and multiple dilution method.Strawberries were sprayed with 200 μg·mL^-1 potassium sorbate, 50 μg·mL^-1 and 100 μg·mL^-1 antimicrobial peptide AMP-17 and stored in refrigerator at 4 ℃.The quality indexes and physicochemical indexes of strawberry during storage were studied, in order to provide a reference for strawberry preservation.The results showed that the MIC（minimal inhibitory concentration） and MBC（minimum bactericidal concentration） of AMP-17on Aspergillus niger,Aspergillus flavus and other fungi were 50μg· mL^-1,100μg· mL^-1,respectively.It was found from the experiment that the AMP-17 had no obvious antibacterial effect on bacteria.Besides,compared with control group,the test groups sprayed with AMP-17 at concentrations of 50μg·mL^-1 and 100μg·mL^-1 could significantly reduce the probability of strawberry spoilage due to the fungal contamination at 4℃.Besides,it also could delay the decrease of vitamin C,titratable acid content and inhibit the increase of total sugar content in strawberries.The impacts of color,appearance,and smell were significantly higher（P＜0.05）than 200 mg·mL^-1 potassium sorbate and control group.