Abstract: The tissue culture seedlings of Lilium brownii var.viridulum induced by bulbs of Wanzai county were used as test material.The environmental factors such as temperature, humidity, and light on the survival rate were analyzed, in order to clarify the influence of environmental factors on the survival rate of virus-free tissue culture seedlings of Lilium brownii var.viridulum.The results showed that, 1) in a single environmental factor, the survival rate of Lilium brownii var.viridulum increased first and then decreased with the increase of test temperature and humidity.When the temperature was higher than 25 ℃,the survival rate reached more than 87.0%.The humidity was 70% to 80%,which was beneficial to the survival of tissue culture seedlings.With the increase of the experimental light intensity, the survival rate was increased.Although the light system of tissue culture seedlings was weak, strong light could the development of photosynthetic systems and the survival rate.2) Environmental factors interactively affect the survival rate.Temperature was the main factor for the survival rate of Lilium brownii var.viridulum,followed by humidity and light intensity in order.The optimal combination of experimental environmental factors was ‘temperature 25 ℃+humidity 85%+light intensity 30%’‘temperature 25 ℃+humidity 75%+light intensity 30%’.3) The survival rate in the whole year was 20.0% to 95.0%,and the peak survival rate appears in March and October.It was not suitable for transplanting in July and August.Tissue culture seedlings of Lilium brownii var.viridulum should be transplanted as early as possible in the first half of the year.Therefore, Lilium brownii var.viridulum tissue culture seedlings should be transplanted as early as possible in the first half of the year.The temperature was above 25 ℃,substrate humidity was above 70%,and non-shading treatment can effectively improve the survival rate.