Abstract: ‘Ningou’,Mengyuan and ‘Nongda No.7’ were used as experimental materials, and the extraction effects of six extraction solvents under four extraction methods on photosynthetic pigments including chlorophyll a, chlorophyll b and carotenoid were compared and analyzed, the optimal extraction scheme for photosynthetic pigment in leaves of three varieties of Chinese dwarf cherry was screened out, in order to provide a reference for the study of photosynthetic physiology, variety breeding and resistance physiology of Cerasus humilis.The results showed that among the four extraction methods, grinding method had the highest extraction efficiency, and the optimum extraction reagent C（acetone∶ethanol 1∶1） was selected.The dry sample extraction method had low efficiency, but it was less affected by the variety.The optimum extraction reagent B（95% ethanol） was selected.The extraction efficiency of fresh sample extraction method was higher, but it was influenced by the resistance of varieties.The pigment was degraded under liquid nitrogen method during storage and grinding, and the content was low.