Abstract: Crossbreeding was carried out by using oriental lily ’Siberia’ ’Martinique’ and ’After Eight O’clock’ as the materials on the basis of investigating the effect of different storage duration on their pollen viability,and the reciprocal crosses were conducted to compare their fruiting rates and embryonic rates.In addition,the effects of different hybridization combinations,developing-embryo age,culture methods and media on the growth and germination of young embryos were compared through embryo rescue technology in overcoming post-fertilization obstacles,in order to construct a scheme suitable for the reciprocal crosses and embryo rescue of oriental lily ’Siberia’ and cultivate a new germplasm of oriental lily.The results showed that there existed fertilization barriers between them,while both inflated ovaries and embryonic seeds were obtained in all of them.Among them,the embryonic rate in the hybridization combinations using ’Siberia’ as the male was significantly higher than that in the female ’Siberia’.In the combination of ’Siberia’ and ’After Eight O’ clock’,the embryonic rale of using ’Siberia’ as the male was 4.5 times that of the female,while in the ’Siberia’ ×’Martinique’ combination,the embryonic rate was 2.3 times that of the female.In the ’Siberia ×Bonaire’ combination,the embryonic rate was 1.01 times that of the female,in the ’Siberia× Sunshine Iceland’ combination,the embryonic rate was 1.2 times that of the female.According to the embryo rescue results,it showed that the 70 days embryos were the most suitable for stripping endosperm culture.In the ’Siberia’ × ’Sunshine Iceland’ hybrid combination,the germination rate of the stripped endosperm culture was the highest among them,which was 5.5 times than that of the unscathed endosperm culture.The optimal medium for embryo culture in vitro was MS+1.0 mg·L^-1 6-BA+0.1 mg · L^-1 NAA+0.3% sucrose.After hybridization,the seeds began to germinate around 20 days of sowing,the germination rate was 0.8%-8.7%,and the emergence rate was significantly increased around 40 days,of which the highest in the ’Siberia’ × ’Bonaire’ hybrid combination was as high as 64.3%.