Abstract: Taking the leaves of ‘Xuxiang’ kiwifruit as the test material, using the in vitro plant tissue culture technology, the effects of disinfection time and different concentrations of phytohormone combinations at different stages in the process of using leaf tissue culture were studied, in order to provide reference for shortening ‘Xuxiang’ tissue culture.The results showed that it took about 70 days for the 6 processes including leaf disinfection, medium selection, callus induction, adventitious bud induction, rooting induction, and seedling transplanting.After collecting the leaves of ‘Xuxiang’ kiwifruit, clean water was needed to wash the epidermis of the leaves for 30-60 minutes.The best disinfection effect was as follows, sterilize with 75% C₂H₅OH in a sterile workbench for 20 seconds, and then use 0.1% HgCl₂ solution for 5 minutes.The survival rate of seedlings was 86.7%.The leaves were cultured in MS+sugar 30 g·L^-1+agar 4.5 g·L^-1+TDZ 2.0 mg·L^-1+IBA 0.5 mg·L^-1 medium for 35 days to obtain the best callus induction effect, and the callus rate was 100%.The optimal medium for callus induction and differentiation of adventitious buds was MS+sugar 30 g·L^-1+agar 4.5 g·L^-1+NAA 0.3 mg·L^-1+6-BA 3.0 mg·L^-1,with a differentiation rate of 93.33%±2.66% and an average number of buds of 16.97±0.79.The best rooting medium was 1/2 MS+sugar 30 g·L^-1+agar 4.5 g·L^-1+IBA 0.8 mg·L^-1+AC 0.25 g·L^-1,the rooting rate was 91.37%±6.35%;the average root length was（7.24±1.04） cm, the average number of roots was 14.46±1.56.After the seedlings were refined for 3-5 days, they were transplanted into soil composed of garden soil, humus soil, vermiculite, perlite, and plant ash（3∶1∶1∶1∶1∶1）.The plant survival rate was higher and the growth was better.With tissue culture technology, a large number of ‘Xuxiang’ seedlings that do not carry pests can be obtainedin a short period of time.