Abstract: Taking pepper as test material, a seed soaking treatment method was used, the effects of different concentrations of vitamin B₆ solution on pepper seed germination under 4 ℃ low temperature treatment and seedling growth under 12 ℃ low temperature treatment were studied, in order to provide reference for further study of _vitamin B₆ induced resistance and regulation mechanism of pepper.The results showed that the germination rate, germination potential and germination index of pepper seeds soaked ⁱⁿ 100 mg·L_<sup>-1 vitamin B₆ solution under 4 ℃ low temperature stress were significantly higher than those of the control; the pepper seed^{s s}oaked in ₁00 mg·L^-1 vitamin B₆ solution grew to have two leaves.The malo_n^dialdehyde content and ■ production rate of pepper seedlings treated at 12 ℃decreased significantly, and the root activity increased; the pe^pper seeds s_oaked in 80 mg·L^-1 vitamin B₆ solution grew to two leaves, and the root length, plant height and fresh weight of the seedlings were higher than that of the control group after being treated at 12 ^℃;the peppe_r seeds soaked in 40 mg·L^-1 vitamin B₆ solution grew to two leaves, and the activities of CAT,POD and SOD,and the root activity of pepper seedlings were increased after being treated at 12 ℃.Therefo_re, the appropriate concentration of vitamin B₆ solution could help to reduce the damage of low temperature to pepper seed germination and seedling growth, and improve its low temperature resistance.