Abstract: Taking the rose cultivar ‘Jinfenghuang’ cuttings as test materials, physiological index determination and membership function analysis method were used, the effects of different concentrations of salicylic acid（SA） on the physiological indexes of rose under low temperature stress were studied, in order to provide reference for popularized and applied in the cultivation and production of rose seedlings.The results showed that spraying SA under low temperature stress could alleviate the decline rate of relative water content and chlorophyll in rose seedling leaves, inhibit the excessive increase of malondialdehyde（MDA） content and relative conductivity, meanwhile, it was beneficial to increase the content of cell osmotic regulator proline（Pro）,soluble sugar and soluble protein; although low temperature stress could induce a large amount of hydrogen peroxide（H₂O₂） in the leaves of rose seedlings, exogenous SA could inhibit the accumulation of H₂O₂ by increase the activities of superoxide dismutase（SOD）,peroxidase（POD） and catalase（CAT）,and the content of ascorbic acid（ASA） at low temperature stress.In conclusion, external application of SA could alleviate the peroxidative damage of rose seedling leaves, and enhance its buffering effect under low temperature stress, and then improve its low temperature tolerance.In particular, it was analyzed by membership function method, the best effect of spraying was from 150 mg·L^-1 SA.