Abstract: The genetic diversity and genetic relationship of 29plant materials of Lespedeza were studied by inter-simple sequence repeat（ISSR） molecular marker technology,and fingerprints were constructed,in order to provide reference for the introduction and breeding of Lespedezain North China.The results showed that a total of 10 polymorphic ISSR primers were screened,PCR amplification was performed by polymorphic ISSR primers,and a total of 93bands were amplified,included 92polymorphic bands,and the polymorphism ratio（PPB）was 98.92%,and primer UBC-812was selected to construct a fingerprint.The average effective gene number was 1.477 5and the average Nei′s genetic diversity index（H）was 0.293 3.Through UPGMA cluster analysis,when the genetic similarity coefficient was 0.61,the 29 materials could be divided into 3 groups,which was not completely consistent with the traditional classification.In conclusion,the genetic diversity of Lespedeza was high,and the selected varieties of Lespedeza bicolor were not completely clustered,indicated that the genetic diversity of Lespedeza bicolor cultivated by cross-breeding and other methods had been significantly improved.