Abstract: Taking ‘Hill’‘Xiangling’‘Robert Livermore’ and 7 ‘Robert Livermore’ natural hybrid F₁ superior plants as experimental materials, a total of 10 walnut varieties（superior plants） were analyzed for genetic diversity using SRAP molecular marker technology, in order to reveal the genetic diversity of the F₁ generation of natural hybrid red walnut and provide reference for the breeding of new red walnut varieties.The results showed that a total of 120 bands were amplified from the 25 pairs of selected primers, and the polymorphism ratio was 80%.The average Nei′s genetic diversity index and Shannon information index were 0.271 0 and 0.406 5,indicating that the genetic diversity and genetic difference among the 10 walnut varieties（superior tree） were relatively rich.The genetic similarity coefficient of the 10 walnut varieties（superior tree） ranged from 0.591 7 to 0.808 3.Ten walnut varieties（superior tree） were divided into three categories when genetic similarity coefficient threshold of 0.704 were selected.‘Xiangling’ was classified into a separate class and had a distant relation with the other 9 walnut varieties（superior tree）.Among the F₁ generation of ‘Robert Livermore’,those with red seed coat color were clustered into one category, while those with yellow seed coat color were clustered into another category.