Abstract: Auricularia heimuer is an indispensable variety in the edible fungus industry in China, and its molecular biology experimental research is increasingly hot, especially with the rapid development of molecular biology technologies such as transcriptome, proteomics, genomics, bioinformatics and so on, molecular marker technology has been widely used in Auricularia heimuer research.In this study, the characteristics and applications of DNA molecular markers were summarized, and the application status of DNA molecular markers（ITS,SSR,ISSR,SRAP） in the genetic diversity and genetic relationship analysis of Auricularia heimuer and the construction of genetic map were reviewed.The problems of DNA molecular markers of Auricularia heimuer were summarized, and prospected the development trend and application prospect in the future.