Abstract: Taking Auricularia heimuer DL202 as the test material, Mn-SOD gene was cloned by RT-PCR,and its bioinformatics analysis and expression analysis were studied, in order to lay the foundation for further study of its function in the stress resistance of A.heimuer.The results showed that Mn-SOD cDNA contained 609 bp and encoded 202 amino acids, named Ah-MnSOD.The amino acid sequence had Mn-SOD conserved domains and had no transmembrane structure and signal peptide.Subcellular localization showed that Ah-MnSOD was modified and synthesized in peroxisome.Phylogenetic analysis indicated that Ah-MnSOD had the closest relationship with Auricularia subglabra.In addition, the prokaryotic expression vector pET-32 a-Ah-MnSOD was constructed and the target protein was successfully expressed by induction with IPTG.The results of qRT-PCR showed that Ah-MnSOD gene was all expressed during different stages of A.heimuer,and reached the highest level at the primordium stage.