Virus Detections and Fruit Quality Analysis on Micro-propagation Seedlings of Strawberry
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摘要: 以2种不同大小的草莓茎尖分生组织为试材，进行组织培养，分别获得再生小植株并进行病毒检测，利用上述脱毒母株和农家自留种苗分别繁育匍匐茎子苗，研究了2种子苗的病毒发生率与果实品质，以期通过草莓脱毒试验为脱毒苗的应用推广提供参考依据。结果表明：不同草莓品种的病毒含量有差异，并且病毒外壳蛋白表达量与其核酸转录量一致。切取草莓匍匐茎约5 mm茎尖分生组织进行第一次脱毒，其部分再生小植株仍然携带了低浓度病毒。与之相比，再切取试管苗约0.5 mm茎尖组织进行第二次脱毒，其再生率虽然仅为31%,但是二次脱毒株均为无毒苗。利用已鉴定的脱毒母株和农家自留种苗分别繁育匍匐茎子苗，经过定植后发现2种子苗的病毒病发生率差异极显著。二次脱毒母株繁育的子苗果实可滴定酸含量显著低于农家自留种苗(P<0.05),而可溶性总糖等品质参数差异不显著。综上所述，以0.5 mm茎尖分生组织为外植体进行草莓组织培养，可以获得优质的脱毒母株，相关研究成果有助于未来草莓良繁体系建设。Abstract: Taking two types of seedlings using large or small sizes explants by meristem tissues culture, obtaining regenerated plantlets were detected for Strawberry mild yellow edge virus.The virus-free mother plants and virus-undetermined control were propagated to produce daughter plants using stolon.Then virus incidences and fruit quality between two kinds of daughter plants were investigated, respectively, in order to provide reference for the virus-free seedlings application in future.The virus elimination studies would be useful for virus-free seedlings application in future.The results showed that virus concentration differences in plants were found between different cultivars, and virus coat protein expression was consistent with its transcription level.Meristem tissues using explant in size of 5 mm in stolon were induced to adventitious buds and then differentiated to in vitro plantlets at the first round of virus elimination.However, a few in vitro plantlets regenerated from explant in size of 5 mm were still infected by lower virus.In comparison, regeneration ratio at the second round of meristem tissues culture using explant in size of 0.5 mm was only 31%.Besides, all seedlings regenerated from the second of meristem culture were found virus free.Two types of daughter plants propagated using stolon from virus-free mother plants and virus-undetermined control were planted at an experimental plot, respectively.The SMYEV incidences were significantly different between two types of daughter plants.The titratable acidity was significantly lower in virus-free daughter plants than control.In conclusion, the virus-free mother plants were successfully produced by meristem tissues culture using explants in sized of 0.5 mm, which could benefit for strawberry propagation program in future.
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