Abstract: Taking two-year-old ‘Sunshine Rose’ grape as test material, 3-4 buds were left for pruning after one-time fruit ripening and harvesting.Three treatments were adopted, no application of cyanamide to break dormancy and remove all leaves of the plant, application of cyanamide to break dormancy and remove all leaves of the plant, and no treatment as control.The germination rate, flower bud differentiation rate, fruiting branch rate and fruit quality were investigated, and the fruit quality of different treatments were measured at the fruit ripening stage,in order to provide reference for improving flower bud differentiation and fruit quality of‘Sunshine Rose’second fruit in dry and hot valley area.The results showed that the second fruit germination rate,flower bud differentiation rate,branching rate,fruiting branch rate,cluster length,cluster width,single fruit weight,single cluster weight,yield and soluble solids content of ‘Sunshine Rose’grape were significantly improved by removing all leaves of the plant.The treatment with single cyanamide to break the sleep and remove all the leaves of the plant had the best effect on the germination and flower bud differentiation of the second fruit of‘Sunshine Rose’grape,and the fruit quality improvement was also the best.