Abstract: Str1,a wild macrofungus collected from Yuanjiang City, was identified by morphology and molecular biology to clarify its taxonomic status.Single factor test was used to study the solid culture conditions of mycelia, and the liquid fermentation capacity of the strain was optimized by adding jute powder, in order to provide reference for the further study of Stropharia rugosoannulata,and also be conducive to the recycling of jute by-products.The results showed that Str1 strain and Stropharia rugosoannulata（Stropharia rugosoannulata）,serial number MF 919 477.1,converged into one branch.Str1 was identified as macroglobularis by morphological characteristics.The optimal temperature for mycelial growth was 25 ℃,pH was 7,the optimal carbon source was glucose, and the optimal nitrogen source was yeast powder.Adding 0.10% jute powder to the liquid medium for shaking fermentation for 7 days could significantly increase the mycelial biomass and the secretion of xylanase, but had little effect on the secretion of carboxymethyl cellulase.