Abstract: The effects of different bactericides and bactericides combinations on the low temperature and cold damage defense of overwintering tomato were studied by using the medium taste and strain resistance tomato ‘Fruit Water’ as the test material, in order to improve the adaptability, cold resistance and fruit quality of overwintering tomato.The results showed that, T3（Rhizome 8+Zorrun microbial agent） treatment and T5（Rhizome 8+Bacillus subtilis） treatment could promote the growth and dry matter accumulation of tomato seedlings, increase the defense enzyme activity and soluble protein content in tomato leaves, and significantly reduce the cold damage index.It could effectively improve the resistance potential of overwintering tomato to cold damage.In tomato fruit quality, according to the average membership function value analysis, the overall ranking was T1（Zorun microbial microbial agent） ＞T5（Grass root 8+Bacillus subtilis） ＞T3（Grass root 8+Zorun microbial microbial agent） ＞T4（Grass root 8+Hartz microbial microbial agent 1） ＞CK（Clean water control） ＞T2（Hartz microbial microbial agent 1） ＞T6（One billion live bacteria soil repair bacterial agent）.In conclusion, T5（Root 8+Bacillus subtilis） treatment had a good effect on improving the resistance and quality of overwintering stubble tomatoes, and could be popularized and applied in overwintering stubble tomato planting.