Abstract: The full length of CcMYB8 gene was cloned from the leaves of Cymbidium with variegated leaf（Cymbidium tracyanum var.‘huanghua’×C.iridioides D.Don.‘Yeyi’） by PCR,and its sequence was analyzed by bioinformatics, amino acid homology and genetic relationship, the function of the CcMYB8 gene were studied, in order to explain the regulation mechanism of R2 R3-MYB subfamily transcription factors on leaf spot formation.The results showed that the CcMYB8 gene of Cymbidium with variegated leaf was an unstable hydrophilic protein with a full length of 657 bp, encodes 219 amino acids, a molecular weight of 24.974 kDa and two SANT conserved domains, and was highly homologous to Cymbidium sinense,with a similarity of 99.54%.The phylogenetic tree also showed that it was closely related to Cymbidium sinense,so the function of CcMYB8 gene is similar or the same as that of this species.