Abstract: Taking 28 cherry resource varieties imported from Qinghai Province as experimental materials, using the methods of indoor isolated leaf inoculation and field live leaf inoculation, the effect of leaf spot disease on 28 introduced resource varieties was studied, in order to screen out the strong disease resistance and provide a reference for the selection and breeding of leaf spot disease resistant varieties in Qinghai Province.The results showed that the inoculation results of isolated leaves in the room and the live leaves in the field were highly consistent with the cluster analysis using Wald′s method and Euclidean distance.Among the 28 cherry cultivars, ‘Early Robin’‘Lambert’and ‘Moreau’ were the stronger resistances of cherry cultivars, while the lower resistances of cherry cultivars were ‘Rainier’‘Tieton’‘Sunburst’ and ‘Hongdeng’.