Abstract: Taking tomato as test material, a randomized block experiment was conducted with 7 treatments, no fertilization（CK1）,local conventional fertilization（CK2）,100% conventional fertilization+bio-organic fertilizer（T1）,90% conventional fertilization+bio-organic fertilizer（T2）,80% conventional fertilization+bio-organic fertilizer（T3）,70% conventional fertilization+bio-organic fertilizer（T4）,60% conventional fertilization+bio-organic fertilizer（T5）.The effects of partial substitution of bio-organic fertilizer for chemical fertilizer on tomato yield, output input ratio, product quality, plant nutrient distribution and fertilizer utilization were studied, in order to provide reference for rational fertilization and improving quality of tomato in solar greenhouse.The results showed that compared with single chemical fertilizer, combined application of bio-organic fertilizer could significantly increase the weight of single fruit and yield per 667 m~2,and improve the utilization rate and absorption of nitrogen, phosphorus and potassium; compared with 100% conventional fertilization, 30% reduction of chemical fertilizer combined with bio-organic fertilizer improved the weight, yield, quality and absorption and utilization rate of nitrogen, phosphorus and potassium of tomato; from the distribution rate of nitrogen, phosphorus and potassium in different parts of the plant, the distribution rate of stem and leaf was the highest, followed by fruit and root.Therefore, reducing the application rate of common chemical fertilizer by 30% and applying 400 kg bio-organic fertilizer per 667 m~2 could increase tomato yield, improve product quality and significantly improve the utilization rate of nitrogen, phosphorus and potassium fertilizer.