Abstract: The fresh fruits of two varieties of Lycii Fructus ‘Yuanguo’（Y） and ‘Ningqi No.7’（Q） stored at 0 ℃ for 36 days were used as test materials to study the effects of differential metabolites produced during storage at 0 ℃ on the storage and preservation of fresh fruits of two varieties of Lycii Fructus, in order to provide reference for prolonging the storage period of ‘Yuanguo’（Y）.The results showed that under the same storage conditions, the significant differential metabolites of ‘Ningqi No.7’（Q） stored at 0 ℃ were mainly phosphate ester, ribose phosphate, glucuronic acid, threonine, and arginine phosphate.The differential metabolites in the fresh fruits of ‘Yuanguo’（Y） were mostly unsaturated fatty acids such as arachidonic acid, eicosatrienoic acid and linolenic acid.These substances were beneficial to ‘Yuanguo’（Y） to resist low temperature stress during storage and prolong its storage period.