Abstract: ’Zhuyan No.1’ is a new cultivar of Pleurotus,breeding the new fine strain by the technique of monosporous hybridization, which choose Henan Province main cultivar ‘CCEF89’ and the sequence of naturalized strain ‘4195’ as the parenrts.The production cycle is 45-50 days.Low and medium temperature varieties, fruiting bodies are superimposed, the pileus is fan-shaped, color is affected by temperature, dark black at low temperature, dark gray at high temperature.The length of stalk is about 5 cm.The diameter of stalk is about 1.5 cm.The pileus diameter and thickness of six or seven mature fruiting bodies are about 5.5 cm and 1.0 cm respectively.The products are uniform and the pileus is thick and tough, and have good commercial properties.The results of regional trials of 2017—2018 showed that the biological efficiency was as high as 84.2%,which was 6.65% higher than control variety ‘Heikang650’.Production test results showed that the biological efficiency was as high as 89.7%,which was an increase of 9.5% over the control variety ‘Heikang650’.Suitable for autumn and wintetr cultivation.