Abstract: Taking the alfalfa variety ‘WL525HQ’ as the test material, a single factor completely random design was adopted, with 6 different cultivation substrate ratios and 2 different planting methods set.By measuring its germination rate, aboveground and underground biomass, and root morphology, suitable substrates and planting methods for the cultivation of alfalfa micro vegetables were screened.Taking 28 alfalfa varieties as test materials, a single factor completely random design was used to screen the best alfalfa varieties for micro vegetable production by measuring saponin content and concanavaline content.By analyzing the correlation between the content of concanavaline and the company, place of origin, and autumn dormancy level through grey relational analysis, in order to provide reference for screening the substrate, planting methods, and varieties of alfalfa micro vegetables for production.The results showed that aboveground and underground biomasses of alfalfa micro-green were the highest under the growing medium, which was a mixture of peat and vermiculite with a volume ratio of 1.Direct sowing could lead to better growth of alfalfa than transplanting.Cultivar ‘WL363HQ’ was the most suitable variety for alfalfa micro-green production due to the lowest canavanine content among 28 alfalfa varieties.Canavanine content was correlated with producing place and fall dormancy rating, which could be used for future variety screening.