Abstract: Polygala fallax Hemsl collected from Lingchuan in Guilin was used as the test material.The tissue block separation, ITS sequencing and plate sputum method were performed to study the composition and antifungi activity of endophytic fungi of Polygala fallax Hemsl, in order to provide reference for the development of biocontrol fungi.The results showed that a total of 62 isolates of endophytic fungi were obtained.They were classified into 29 different genus according to their ITS characters, and Colletotrichum sp.,Diaporthe sp.,Fusarium sp.,Phomopsis sp.were the dominant genus.Ten isolates showed antifungi activity against Mycosphaerella fijiensis,Diaporthe citri（Fawcett） wolf, Phyllosticta zingiberi.The medicinal plant Polygala fallax Hemsl showed relatively abundant endophytic fungal resource for pathogenic inhibition, and its antifungi inhibition was worthwhile for further study.